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Quantification of Bacterial Twitching Motility in Dense Colonies Using Transmitted Light Microscopy and Computational Image Analysis

A method was developed to allow the quantification and mapping of relative bacterial twitching motility in dense samples, where tracking of individual bacteria was not feasible. In this approach, movies of bacterial films were acquired using differential interference contrast microscopy (DIC), and b...

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Bibliographische Detailangaben
Veröffentlicht in:Bio Protoc
Hauptverfasser: Smith, Benjamin, Li, Jianfang, Metruccio, Matteo, Wan, Stephanie, Evans, David, Fleiszig, Suzanne
Format: Artigo
Sprache:Inglês
Veröffentlicht: Bio-Protocol 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5951402/
https://ncbi.nlm.nih.gov/pubmed/29770353
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2804
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