Live-cell analysis of endogenous GFP-RPB1 uncovers rapid turnover of initiating and promoter-paused RNA Polymerase II

Initiation and promoter-proximal pausing are key regulatory steps of RNA Polymerase II (Pol II) transcription. To study the in vivo dynamics of endogenous Pol II during these steps, we generated fully functional GFP-RPB1 knockin cells. GFP-RPB1 photobleaching combined with computational modeling rev...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Steurer, Barbara, Janssens, Roel C., Geverts, Bart, Geijer, Marit E., Wienholz, Franziska, Theil, Arjan F., Chang, Jiang, Dealy, Shannon, Pothof, Joris, van Cappellen, Wiggert A., Houtsmuller, Adriaan B., Marteijn, Jurgen A.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2018
Assuntos:
PNAS Plus
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5948963/
https://ncbi.nlm.nih.gov/pubmed/29632207
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1717920115
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