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Live-cell analysis of endogenous GFP-RPB1 uncovers rapid turnover of initiating and promoter-paused RNA Polymerase II
Initiation and promoter-proximal pausing are key regulatory steps of RNA Polymerase II (Pol II) transcription. To study the in vivo dynamics of endogenous Pol II during these steps, we generated fully functional GFP-RPB1 knockin cells. GFP-RPB1 photobleaching combined with computational modeling rev...
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Publicado no: | Proc Natl Acad Sci U S A |
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Main Authors: | , , , , , , , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
National Academy of Sciences
2018
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5948963/ https://ncbi.nlm.nih.gov/pubmed/29632207 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1717920115 |
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