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Microplate Chemiluminescent Assay for DNA Detection Using Apoperoxidase-Oligonucleotide as Capture Conjugate and HRP-Streptavidin Signaling System

A covalent conjugate of horseradish apoperoxidase and amino-containing oligonucleotide was synthesized for the first time. Using the obtained conjugate as a capture reagent chemiluminescent microtiter plate-based assay for detection of 35-mer fragment of hepatitis B virus (HBV) DNA (proof-of-concept...

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Detalhes bibliográficos
Publicado no:Sensors (Basel)
Autor principal: Sakharov, Ivan
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5948693/
https://ncbi.nlm.nih.gov/pubmed/29690600
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/s18041289
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