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Monovalent Streptavidin that Senses Oligonucleotides

We report a straightforward chemical route to monovalent streptavidin, a valuable reagent for imaging. The one-step process is based on a (tris)biotinylated-oligonucleotide blocking three of streptavidin’s four biotin binding sites. Further, the complex is highly sensitive to single-base differences...

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Detalhes bibliográficos
Main Authors: Taylor, Steven K., Wang, Jingxian, Kostic, Natasa, Stojanovic, Milan N.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4109606/
https://ncbi.nlm.nih.gov/pubmed/23606329
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201209948
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