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High‐capacity protein A affinity chromatography for the fast quantification of antibodies: Two‐wavelength detection expands linear range

The high‐throughput analysis of antibodies from processes can be enhanced when the linear range is expanded and sample preparation is kept to a minimum. We developed a fast chromatography method based on a hexameric variant of staphylococcal protein A immobilized on Toyopearl matrix, TSK 5 PW using...

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Bibliographic Details
Published in:J Sep Sci
Main Authors: Satzer, Peter, Jungbauer, Alois
Format: Artigo
Language:Inglês
Published: John Wiley and Sons Inc. 2018
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5947708/
https://ncbi.nlm.nih.gov/pubmed/29330912
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jssc.201701481
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