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Quantifying Reversible Surface Binding via Surface-Integrated Fluorescence Correlation Spectroscopy

[Image: see text] We present a simple and versatile single-molecule-based method for the accurate determination of binding rates to surfaces or surface bound receptors. To quantify the reversible surface attachment of fluorescently labeled molecules, we have modified previous schemes for fluorescenc...

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Detalhes bibliográficos
Publicado no:Nano Lett
Main Authors: Mücksch, Jonas, Blumhardt, Philipp, Strauss, Maximilian T., Petrov, Eugene P., Jungmann, Ralf, Schwille, Petra
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2018
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5946168/
https://ncbi.nlm.nih.gov/pubmed/29658275
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.nanolett.8b00875
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