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A Riboswitch-based Inducible Gene Expression System for Trypanosoma brucei
Generation of conditional mutants in Trypanosoma brucei can be done by the use of RNA interference (RNAi). However, RNAi frequently produces off target effects. Here we present an alternative strategy in which the glmS ribozyme is inserted in the C-terminal region of one allele of a GOI and effectiv...
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| Publicat a: | J Eukaryot Microbiol |
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| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2018
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5935546/ https://ncbi.nlm.nih.gov/pubmed/29265590 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/jeu.12493 |
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