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A Riboswitch-based Inducible Gene Expression System for Trypanosoma brucei

Generation of conditional mutants in Trypanosoma brucei can be done by the use of RNA interference (RNAi). However, RNAi frequently produces off target effects. Here we present an alternative strategy in which the glmS ribozyme is inserted in the C-terminal region of one allele of a GOI and effectiv...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:J Eukaryot Microbiol
Prif Awduron: Cruz-Bustos, Teresa, Ramakrishnan, Srinivasan, Cordeiro, Ciro D., Ahmed, Michael A., Docampo, Roberto
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2018
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5935546/
https://ncbi.nlm.nih.gov/pubmed/29265590
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/jeu.12493
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