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Propagating Gateway Vectors
Generating stocks of Entry and Destination vectors for use in the Gateway recombinatorial cloning system requires transforming them into Escherichia coli strain DB3.1, where they can replicate because this strain is immune to the effects of the ccdB gene carried in the Gateway cassette. However, mut...
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| Pubblicato in: | Cold Spring Harb Protoc |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2018
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5934992/ https://ncbi.nlm.nih.gov/pubmed/29295904 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot094920 |
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