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An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes
Electroporation of zygotes represents a rapid alternative to the elaborate pronuclear injection procedure for CRISPR/Cas9-mediated genome editing in mice. However, current protocols for electroporation either require the investment in specialized electroporators or corrosive pre-treatment of zygotes...
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| Veröffentlicht in: | PLoS One |
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| Hauptverfasser: | , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Public Library of Science
2018
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5933690/ https://ncbi.nlm.nih.gov/pubmed/29723268 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0196891 |
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