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Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
Sectioning via paraffin embedding is a broadly established technique in eukaryotic systems. Here we provide a method for the fixation, embedding, and sectioning of intact microbial colony biofilms using perfused paraffin wax. To adapt this method for use on colony biofilms, we developed techniques f...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5933251/ https://ncbi.nlm.nih.gov/pubmed/29630036 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57196 |
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