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Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

Sectioning via paraffin embedding is a broadly established technique in eukaryotic systems. Here we provide a method for the fixation, embedding, and sectioning of intact microbial colony biofilms using perfused paraffin wax. To adapt this method for use on colony biofilms, we developed techniques f...

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Dades bibliogràfiques
Publicat a:J Vis Exp
Autors principals: Cornell, William C., Morgan, Chase J., Koyama, Leslie, Sakhtah, Hassan, Mansfield, Jennifer H., Dietrich, Lars E.P.
Format: Artigo
Idioma:Inglês
Publicat: MyJove Corporation 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5933251/
https://ncbi.nlm.nih.gov/pubmed/29630036
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57196
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