Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

Sectioning via paraffin embedding is a broadly established technique in eukaryotic systems. Here we provide a method for the fixation, embedding, and sectioning of intact microbial colony biofilms using perfused paraffin wax. To adapt this method for use on colony biofilms, we developed techniques f...

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Vydáno v:J Vis Exp
Hlavní autoři: Cornell, William C., Morgan, Chase J., Koyama, Leslie, Sakhtah, Hassan, Mansfield, Jennifer H., Dietrich, Lars E.P.
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2018
Témata:
Immunology and Infection
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5933251/
https://ncbi.nlm.nih.gov/pubmed/29630036
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57196
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