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Systematic evaluation of immobilized trypsin based fast protein digestion for deep and high-throughput bottom-up proteomics
Immobilized trypsin (IM) has been recognized as an alternative to free trypsin (FT) for accelerating protein digestion 30 years ago. However, some questions of IM still need to be answered. How does the solid matrix of IM influence its preference for protein cleavage and how well can IM perform for...
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| Publicado no: | Proteomics |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5932237/ https://ncbi.nlm.nih.gov/pubmed/29577644 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.201700432 |
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