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Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
Reporter enzyme fluorescence (REF) utilizes substrates that are specific for enzymes present in target organisms of interest for imaging or detection by fluorescence or bioluminescence. We utilize BlaC, an enzyme expressed constitutively by all M. tuberculosis strains. REF allows rapid quantificatio...
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| Publicado no: | J Vis Exp |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5931367/ https://ncbi.nlm.nih.gov/pubmed/29553533 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56801 |
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