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Simultaneous Real-Time Measurement of the β-Cell Membrane Potential and Ca(2+) Influx to Assess the Role of Potassium Channels on β-Cell Function

Stimulus-secretion coupling in pancreatic β-cells requires Ca(2+) influx through voltage-dependent Ca(2+) channels, whose activity is controlled by the plasma membrane potential (V(m)). Here, we present a method of measuring fluctuations in the β-cell V(m) and Ca(2+) influx simultaneously, which pro...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Vierra, Nicholas C., Dickerson, Matthew T., Philipson, Louis H., Jacobson, David A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5927608/
https://ncbi.nlm.nih.gov/pubmed/29058185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7362-0_7
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