Self-homodimerization of an actinoporin by disulfide bridging reveals implications for their structure and pore formation

The Trp111 to Cys mutant of sticholysin I, an actinoporin from Stichodactyla helianthus sea anemone, forms a homodimer via a disulfide bridge. The purified dimer is 193 times less hemolytic than the monomer. Ultracentrifugation, dynamic light scattering and size-exclusion chromatography demonstrate...

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Bibliografske podrobnosti
izdano v:Sci Rep
Main Authors: Valle, Aisel, Pérez-Socas, Luis Benito, Canet, Liem, Hervis, Yadira de la Patria, de Armas-Guitart, German, Martins-de-Sa, Diogo, Lima, Jônatas Cunha Barbosa, Souza, Adolfo Carlos Barros, Barbosa, João Alexandre Ribeiro Gonçalves, de Freitas, Sonia Maria, Pazos, Isabel Fabiola
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group UK 2018
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5920107/
https://ncbi.nlm.nih.gov/pubmed/29700324
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-24688-2
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