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In situ Localization of the Human Multidrug‐resistance Gene mRNA Using Thymine‐Thymine Dimerized Single‐stranded cDNA

In order to detect the mRNA transcribed from the multidrug‐resistance gene (MDR1), thymine‐thymine (T‐T) dimerized single‐stranded DNA probes have been utilized for hybridization with mRNA either on nitrocellulose filters or in cells and tissues. S1 nuclease digestion rather than sonication was used...

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Publicat a:Jpn J Cancer Res
Autors principals: Sugawara, Isamu, Koji, Takehiko, Ueda, Kazumitsu, Pastan, Ira, Gottesman, Michael M., Nakane, Paul K., Mori, Shigeo
Format: Artigo
Idioma:Inglês
Publicat: Blackwell Publishing Ltd 1990
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5918119/
https://ncbi.nlm.nih.gov/pubmed/1977730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1349-7006.1990.tb02672.x
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