In situ Localization of the Human Multidrug‐resistance Gene mRNA Using Thymine‐Thymine Dimerized Single‐stranded cDNA

In order to detect the mRNA transcribed from the multidrug‐resistance gene (MDR1), thymine‐thymine (T‐T) dimerized single‐stranded DNA probes have been utilized for hybridization with mRNA either on nitrocellulose filters or in cells and tissues. S1 nuclease digestion rather than sonication was used...

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Vydáno v:Jpn J Cancer Res
Hlavní autoři: Sugawara, Isamu, Koji, Takehiko, Ueda, Kazumitsu, Pastan, Ira, Gottesman, Michael M., Nakane, Paul K., Mori, Shigeo
Médium: Artigo
Jazyk:Inglês
Vydáno: Blackwell Publishing Ltd 1990
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5918119/
https://ncbi.nlm.nih.gov/pubmed/1977730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1349-7006.1990.tb02672.x
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