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In vitro synthesis of gene-length single-stranded DNA

Single-stranded DNA (ssDNA) increases the likelihood of homology directed repair with reduced cellular toxicity. However, ssDNA synthesis strategies are limited by the maximum length attainable, ranging from a few hundred nucleotides for chemical synthesis to a few thousand nucleotides for enzymatic...

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Bibliografski detalji
Izdano u:Sci Rep
Glavni autori: Veneziano, Rémi, Shepherd, Tyson R., Ratanalert, Sakul, Bellou, Leila, Tao, Chaoqun, Bathe, Mark
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group UK 2018
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5916881/
https://ncbi.nlm.nih.gov/pubmed/29695837
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-24677-5
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