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Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes

Visualization of DNA for fluorescence microscopy utilizes a variety of dyes such as cyanine dyes. These dyes are utilized due to their high affinity and sensitivity for DNA. In order to determine if the DNA molecules are full length after the completion of the experiment, a method is required to det...

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Foilsithe in:J Vis Exp
Main Authors: Maschmann, April, Masters, Cody, Davison, Melissa, Lallman, Joshua, Thompson, Drew, Kounovsky-Shafer, Kristy L.
Formáid: Artigo
Teanga:Inglês
Foilsithe: MyJove Corporation 2018
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Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5912327/
https://ncbi.nlm.nih.gov/pubmed/29443093
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57141
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