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Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes

Visualization of DNA for fluorescence microscopy utilizes a variety of dyes such as cyanine dyes. These dyes are utilized due to their high affinity and sensitivity for DNA. In order to determine if the DNA molecules are full length after the completion of the experiment, a method is required to det...

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Библиографические подробности
Опубликовано в: :J Vis Exp
Главные авторы: Maschmann, April, Masters, Cody, Davison, Melissa, Lallman, Joshua, Thompson, Drew, Kounovsky-Shafer, Kristy L.
Формат: Artigo
Язык:Inglês
Опубликовано: MyJove Corporation 2018
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5912327/
https://ncbi.nlm.nih.gov/pubmed/29443093
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57141
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