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Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes

Visualization of DNA for fluorescence microscopy utilizes a variety of dyes such as cyanine dyes. These dyes are utilized due to their high affinity and sensitivity for DNA. In order to determine if the DNA molecules are full length after the completion of the experiment, a method is required to det...

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Dettagli Bibliografici
Pubblicato in:J Vis Exp
Autori principali: Maschmann, April, Masters, Cody, Davison, Melissa, Lallman, Joshua, Thompson, Drew, Kounovsky-Shafer, Kristy L.
Natura: Artigo
Lingua:Inglês
Pubblicazione: MyJove Corporation 2018
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5912327/
https://ncbi.nlm.nih.gov/pubmed/29443093
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57141
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