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CRISPR-Trap: a clean approach for the generation of gene knockouts and gene replacements in human cells
CRISPR/Cas9-based genome editing offers the possibility to knock out almost any gene of interest in an affordable and simple manner. The most common strategy is the introduction of a frameshift into the open reading frame (ORF) of the target gene which truncates the coding sequence (CDS) and targets...
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| Publicado no: | Mol Biol Cell |
|---|---|
| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The American Society for Cell Biology
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5909934/ https://ncbi.nlm.nih.gov/pubmed/29167381 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E17-05-0288 |
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