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The impact of storage buffer, DNA extraction method, and polymerase on microbial analysis

Next-generation sequencing approaches used to characterize microbial communities are subject to technical caveats that can lead to major distortion of acquired data. Determining the optimal sample handling protocol is essential to minimize the bias for different sample types. Using a mock community...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Hallmaier-Wacker, Luisa K., Lueert, Simone, Roos, Christian, Knauf, Sascha
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5908915/
https://ncbi.nlm.nih.gov/pubmed/29674641
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-24573-y
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