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Impact of DNA Polymerases and Their Buffer Systems on Quantitative Real-Time PCR

An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed c...

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Autori principali: Wolffs, Petra, Grage, Halfdan, Hagberg, Oskar, Rådström, Peter
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2004
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC321720/
https://ncbi.nlm.nih.gov/pubmed/14715792
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.1.408-411.2004
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