Leukocyte Dynamics Influence Reference Gene Stability in Whole Blood: Data-Driven qRT-PCR Normalization Is a Robust Alternative for Measurement of Transcriptional Biomarkers

BACKGROUND: The use of reference genes for normalization of whole blood qRT-PCR data may be problematic in conditions such as stroke which induce alterations in white blood cell differential. In this study, we assessed the influence of stroke on the stability of commonly employed reference genes, an...

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Detalhes bibliográficos
Publicado no:Lab Med
Main Authors: O’Connell, Grant C, Treadway, Madison B, Petrone, Ashley B, Tennant, Connie S, Lucke-Wold, Noelle, Chantler, Paul D, Barr, Taura L
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2017
Assuntos:
Science
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5907901/
https://ncbi.nlm.nih.gov/pubmed/29069468
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/labmed/lmx035
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