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Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
This protocol demonstrates a method for obtaining high yield and viability for mouse hepatocytes and sinusoidal endothelial cells (SECs) suitable for culturing or for obtaining cell lysates. In this protocol, the portal vein is used as the site for catheterization, rather than the vena cava, as this...
Tallennettuna:
| Julkaisussa: | J Vis Exp |
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| Päätekijät: | , , , , , , |
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
MyJove Corporation
2018
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5894826/ https://ncbi.nlm.nih.gov/pubmed/29553556 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56993 |
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