Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion

This protocol demonstrates a method for obtaining high yield and viability for mouse hepatocytes and sinusoidal endothelial cells (SECs) suitable for culturing or for obtaining cell lysates. In this protocol, the portal vein is used as the site for catheterization, rather than the vena cava, as this...

Ful tanımlama

Kaydedildi:
Detaylı Bibliyografya
Yayımlandı:J Vis Exp
Asıl Yazarlar: Cabral, Fatima, Miller, Colton M., Kudrna, Katrina M., Hass, Blake E., Daubendiek, Jocelyn G., Kellar, Brianna M., Harris, Edward N.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: MyJove Corporation 2018
Konular:
Biochemistry
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC5894826/
https://ncbi.nlm.nih.gov/pubmed/29553556
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56993
Etiketler: Etiketle
Etiket eklenmemiş, İlk siz ekleyin!

Benzer Materyaller