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One‐step collagenase method for zona pellucida removal in unfertilized eggs: easy and gentle method for large‐scale preparation

PURPOSE: Zona pellucida (ZP)‐free eggs are often used for studies such as evaluating the interaction of sperm‐oolemma. To acquire ZP‐free eggs, the most commonly used methods employ acidified Tyrode's solution, enzymatic digestion with a trypsin‐like enzyme, or mechanical methods using micropip...

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Vydáno v:Reprod Med Biol
Hlavní autoři: Yamatoya, Kenji, Ito, Chizuru, Araki, Motoyuki, Furuse, Ryoji, Toshimori, Kiyotaka
Médium: Artigo
Jazyk:Inglês
Vydáno: John Wiley and Sons Inc. 2011
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5892997/
https://ncbi.nlm.nih.gov/pubmed/29662353
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12522-011-0075-8
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