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One‐step collagenase method for zona pellucida removal in unfertilized eggs: easy and gentle method for large‐scale preparation
PURPOSE: Zona pellucida (ZP)‐free eggs are often used for studies such as evaluating the interaction of sperm‐oolemma. To acquire ZP‐free eggs, the most commonly used methods employ acidified Tyrode's solution, enzymatic digestion with a trypsin‐like enzyme, or mechanical methods using micropip...
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| Publicado no: | Reprod Med Biol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
John Wiley and Sons Inc.
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5892997/ https://ncbi.nlm.nih.gov/pubmed/29662353 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12522-011-0075-8 |
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