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FRET-based Stoichiometry Measurements of Protein Complexes in vitro

For a complete understanding of biochemical reactions, information on complex stoichiometry is essential. However, measuring stoichiometry is experimentally challenging. Our lab has developed a FRET-based assay to study protein complex stoichiometry in vitro. This assay, also known as Job plot, is s...

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Bibliografiske detaljer
Udgivet i:Bio Protoc
Main Authors: Mattiroli, Francesca, Gu, Yajie, Luger, Karolin
Format: Artigo
Sprog:Inglês
Udgivet: Bio-Protocol 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5891122/
https://ncbi.nlm.nih.gov/pubmed/29644254
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2713
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