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CRISPR-mediated Tagging with BirA Allows Proximity Labeling in Toxoplasma gondii
Defining protein interaction networks can provide key insights into how protein complexes govern complex biological problems. Here we define a method for proximity based labeling using permissive biotin ligase to define protein networks in the intracellular parasite Toxoplasma gondii. When combined...
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| Publicat a: | Bio Protoc |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Bio-Protocol
2018
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5890295/ https://ncbi.nlm.nih.gov/pubmed/29644258 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2768 |
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