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CRISPR-mediated Tagging with BirA Allows Proximity Labeling in Toxoplasma gondii
Defining protein interaction networks can provide key insights into how protein complexes govern complex biological problems. Here we define a method for proximity based labeling using permissive biotin ligase to define protein networks in the intracellular parasite Toxoplasma gondii. When combined...
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| Publié dans: | Bio Protoc |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Bio-Protocol
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5890295/ https://ncbi.nlm.nih.gov/pubmed/29644258 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2768 |
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