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Characterization of PKACα enzyme kinetics and inhibition in an HPLC assay with a chromophoric substrate

Here we describe a convenient, inexpensive, and non-hazardous method for the measurement of kinase activity of the catalytic subunit of cAMP-dependent protein kinase (PKACα). The assay is based on the separation of a substrate peptide labeled with a strong chromophore from the phosphorylated product...

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Detalhes bibliográficos
Publicado no:Anal Biochem
Main Authors: Luzi, Nicole M., Lyons, Charles E., Peterson, Darrell L., Ellis, Keith C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5889107/
https://ncbi.nlm.nih.gov/pubmed/28595966
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2017.06.001
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