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Characterization of PKACα enzyme kinetics and inhibition in an HPLC assay with a chromophoric substrate
Here we describe a convenient, inexpensive, and non-hazardous method for the measurement of kinase activity of the catalytic subunit of cAMP-dependent protein kinase (PKACα). The assay is based on the separation of a substrate peptide labeled with a strong chromophore from the phosphorylated product...
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| Publicado no: | Anal Biochem |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5889107/ https://ncbi.nlm.nih.gov/pubmed/28595966 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2017.06.001 |
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