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Proximity Biotinylation as a Method for Mapping Proteins Associated with mtDNA in Living Cells

A recurring challenge in cell biology is to define the molecular components of macromolecular complexes of interest. The predominant method, immunoprecipitation, recovers only strong interaction partners that survive cell lysis and repeated detergent washes. We explored peroxidase-catalyzed proximit...

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Detalhes bibliográficos
Publicado no:Cell Chem Biol
Main Authors: Han, Shuo, Udeshi, Namrata D., Deerinck, Thomas J., Svinkina, Tanya, Ellisman, Mark H., Carr, Steven A., Ting, Alice Y.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5886301/
https://ncbi.nlm.nih.gov/pubmed/28238724
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2017.02.002
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