Prediction of Protein Cleavage Sites by the Barley Cysteine Endoproteases EP-A and EP-B Based on the Kinetics of Synthetic Peptide Hydrolysis

Hordeins, the natural substrates of barley (Hordeum vulgare) cysteine endoproteases (EPs), were isolated as protein bodies and degraded by purified EP-B from green barley malt. Cleavage specificity was determined by synthesizing internally quenched, fluorogenic tetrapeptide substrates of the general...

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Detaylı Bibliyografya
Asıl Yazarlar: Davy, Anne, Sørensen, Mikael Blom, Svendsen, Ib, Cameron-Mills, Verena, Simpson, David J.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society of Plant Physiologists 2000
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC58852/
https://ncbi.nlm.nih.gov/pubmed/10631257
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