Structural Characterization and Directed Evolution of a Novel Acetyl Xylan Esterase Reveals Thermostability Determinants of the Carbohydrate Esterase 7 Family

A hot desert hypolith metagenomic DNA sequence data set was screened in silico for genes annotated as acetyl xylan esterases (AcXEs). One of the genes identified encoded an ∼36-kDa protein (Axe1(NaM1)). The synthesized gene was cloned and expressed, and the resulting protein was purified. NaM1 was o...

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Detaylı Bibliyografya
Yayımlandı:Appl Environ Microbiol
Asıl Yazarlar: Adesioye, Fiyinfoluwa A., Makhalanyane, Thulani P., Vikram, Surendra, Sewell, Bryan T., Schubert, Wolf-Dieter, Cowan, Don A.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society for Microbiology 2018
Konular:
Enzymology and Protein Engineering
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC5881061/
https://ncbi.nlm.nih.gov/pubmed/29453256
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02695-17
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