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Structural Characterization and Directed Evolution of a Novel Acetyl Xylan Esterase Reveals Thermostability Determinants of the Carbohydrate Esterase 7 Family
A hot desert hypolith metagenomic DNA sequence data set was screened in silico for genes annotated as acetyl xylan esterases (AcXEs). One of the genes identified encoded an ∼36-kDa protein (Axe1(NaM1)). The synthesized gene was cloned and expressed, and the resulting protein was purified. NaM1 was o...
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| Publié dans: | Appl Environ Microbiol |
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| Auteurs principaux: | , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5881061/ https://ncbi.nlm.nih.gov/pubmed/29453256 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02695-17 |
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