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Ultrasensitive detection and identification of fluorescent molecules by FCS: Impact for immunobiology

An experimental application of fluorescence correlation spectroscopy is presented for the detection and identification of fluorophores and auto-Abs in solution. The recording time is between 2 and 60 sec. Because the actual number of molecules in the unit volume (confocal detection volume of about 1...

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Detalhes bibliográficos
Main Authors: Földes-Papp, Zeno, Demel, Ulrike, Tilz, Gernot P.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC58760/
https://ncbi.nlm.nih.gov/pubmed/11572995
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.181337998
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