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Delivery of the Cre recombinase by a self-deleting lentiviral vector: Efficient gene targeting in vivo

The Cre recombinase (Cre) from bacteriophage P1 is an important tool for genetic engineering in mammalian cells. We constructed lentiviral vectors that efficiently deliver Cre in vitro and in vivo. Surprisingly, we found a significant reduction in proliferation and an accumulation in the G(2)/M phas...

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Autori principali: Pfeifer, Alexander, Brandon, Eugene P., Kootstra, Neeltje, Gage, Fred H., Verma, Inder M.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The National Academy of Sciences 2001
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC58750/
https://ncbi.nlm.nih.gov/pubmed/11553794
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.201415498
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