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Impact of Glycerol as Carbon Source onto Specific Sugar and Inducer Uptake Rates and Inclusion Body Productivity in E. coli BL21(DE3)

The Gram-negative bacterium E. coli is the host of choice for a multitude of used recombinant proteins. Generally, cultivation is easy, media are cheap, and a high product titer can be obtained. However, harsh induction procedures using isopropyl β-d-1 thiogalactopyranoside as inducer are often refe...

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Bibliografiske detaljer
Udgivet i:Bioengineering (Basel)
Main Authors: Kopp, Julian, Slouka, Christoph, Ulonska, Sophia, Kager, Julian, Fricke, Jens, Spadiut, Oliver, Herwig, Christoph
Format: Artigo
Sprog:Inglês
Udgivet: MDPI 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5874867/
https://ncbi.nlm.nih.gov/pubmed/29267215
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/bioengineering5010001
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