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Characterization of the nucleotide-binding capacity and the ATPase activity of the PIP(3)-binding protein JFC1
In this work, we demonstrate that the phosphatidylinositol 3,4,5-trisphosphate-binding protein JFC1 is an ATP-binding protein with magnesium-dependent ATPase activity. We show that JFC1 specifically binds to the ATP analog 8-azido-[α-(32)P]ATP. The affinity of JFC1 for [α-(32)P]ATP was 10× greater t...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The National Academy of Sciences
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC58712/ https://ncbi.nlm.nih.gov/pubmed/11553774 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.191369598 |
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