A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters

We here present a new way to engineer complex proteins toward multidimensional specifications, through a simple yet scalable directed evolution strategy. By robotically picking mammalian cells that are identified, under a microscope, to express proteins that simultaneously exhibit several specific p...

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Veröffentlicht in:Nat Chem Biol
Hauptverfasser: Piatkevich, Kiryl D., Jung, Erica E., Straub, Christoph, Linghu, Changyang, Park, Demian, Suk, Ho-Jun, Hochbaum, Daniel R., Goodwin, Daniel, Pnevmatikakis, Eftychios, Pak, Nikita, Kawashima, Takashi, Yang, Chao-Tsung, Rhoades, Jeffrey L., Shemesh, Or, Asano, Shoh, Yoon, Young-Gyu, Freifeld, Limor, Saulnier, Jessica L., Riegler, Clemens, Engert, Florian, Hughes, Thom, Drobizhev, Mikhail, Szabo, Balint, Ahrens, Misha B., Flavell, Steven W., Sabatini, Bernardo L., Boyden, Edward S.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2018
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5866759/
https://ncbi.nlm.nih.gov/pubmed/29483642
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41589-018-0004-9
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