Improving the efficiency of homologous recombination by chemical and biological approaches in Yarrowia lipolytica

Gene targeting is a challenge in Yarrowia lipolytica (Y. lipolytica) where non-homologous end-joining (NHEJ) is predominant over homologous recombination (HR). To improve the frequency and efficiency of HR in Y. lipolytica, the ku70 gene responsible for a double stand break (DSB) repair in the NHEJ...

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發表在:PLoS One
Main Authors: Jang, In-Seung, Yu, Byung Jo, Jang, Ji Yeon, Jegal, Jonggeon, Lee, Ju Young
格式: Artigo
語言:Inglês
出版: Public Library of Science 2018
主題:
Research Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5864075/
https://ncbi.nlm.nih.gov/pubmed/29566071
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0194954
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