Early MinION(™) nanopore single-molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples

Until recently, the method of choice to characterize viral diversity consisted in cloning PCR amplicons of full-length viral genomes and Sanger-sequencing of multiple clones. However, this is extremely laborious, time-consuming, and low-throughput. Next generation short-read sequencing appears also...

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Publicat a:PLoS One
Autors principals: Sauvage, Virginie, Boizeau, Laure, Candotti, Daniel, Vandenbogaert, Mathias, Servant-Delmas, Annabelle, Caro, Valérie, Laperche, Syria
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2018
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5864009/
https://ncbi.nlm.nih.gov/pubmed/29566006
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0194366
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