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Tracking Endocytosis and Intracellular Trafficking of Epitope-tagged Syntaxin 3 by Antibody Feeding in Live, Polarized MDCK Cells

The uptake and trafficking of cell surface receptors can be monitored by a technique called ‘antibody-feeding’ which uses an externally applied antibody to label the receptor on the surface of cultured, live cells. Here, we adapt the traditional antibody-feeding experiment to polarized epithelial ce...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Giovannone, Adrian J., Reales, Elena, Bhattaram, Pallavi, Fraile-Ramos, Alberto, Weimbs, Thomas
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5857947/
https://ncbi.nlm.nih.gov/pubmed/29564371
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2453
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