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Evolved polymerases facilitate selection of fully 2′-OMe-modified aptamers

RNA or DNA aptamers with 2′-OMe-modifications have been pursued to increase resistance to nucleases, but have been difficult to identify because the OMe groups ablate polymerase recognition. We recently reported evolution of the thermostable DNA polymerases SFM4-6 and SFM4-9, which enable the effici...

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Vydáno v:Chem Sci
Hlavní autoři: Liu, Zhixia, Chen, Tingjian, Romesberg, Floyd E.
Médium: Artigo
Jazyk:Inglês
Vydáno: Royal Society of Chemistry 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5855981/
https://ncbi.nlm.nih.gov/pubmed/29568464
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7sc03747c
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