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Determination of Proteinaceous Selenocysteine in Selenized Yeast

A method for the quantitation of proteinaceous selenocysteine (SeCys) in Se-rich yeast was developed. The method is based on the reduction of the Se-Se and S-Se bridges with dithiotretiol, derivatization with iodoacetamide (carbamidomethylation), followed by HPLC-ICP MS. The chromatographic conditio...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:Int J Mol Sci
Päätekijät: Bierla, Katarzyna, Lobinski, Ryszard, Szpunar, Joanna
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: MDPI 2018
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC5855765/
https://ncbi.nlm.nih.gov/pubmed/29439473
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms19020543
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