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Determination of Proteinaceous Selenocysteine in Selenized Yeast
A method for the quantitation of proteinaceous selenocysteine (SeCys) in Se-rich yeast was developed. The method is based on the reduction of the Se-Se and S-Se bridges with dithiotretiol, derivatization with iodoacetamide (carbamidomethylation), followed by HPLC-ICP MS. The chromatographic conditio...
Enregistré dans:
| Publié dans: | Int J Mol Sci |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
MDPI
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5855765/ https://ncbi.nlm.nih.gov/pubmed/29439473 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms19020543 |
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