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The phorbol 12-myristate-13-acetate differentiation protocol is critical to the interaction of THP-1 macrophages with Salmonella Typhimurium

THP-1 cells differentiated with phorbol 12-myristate 13-acetate (PMA) are widely used as a model for function and biology of human macrophages. However, the conditions used for differentiation, particularly the concentration of PMA and the duration of treatment, vary widely. Here we compare several...

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Udgivet i:PLoS One
Main Authors: Starr, Tregei, Bauler, Timothy J., Malik-Kale, Preeti, Steele-Mortimer, Olivia
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5851575/
https://ncbi.nlm.nih.gov/pubmed/29538403
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0193601
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