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Construction of Designer Selectable Marker Deletions with a CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and New Design of Common Entry Vectors
Vectors encoding selectable markers have been widely used in yeast to maintain or express exogenous DNA fragments. In the fission yeast Schizosaccharomyces pombe, several engineered markers have been reported and widely used, such as ura4(+) and ScLEU2 from Saccharomyces cerevisiae, which complement...
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| Publicado no: | G3 (Bethesda) |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Genetics Society of America
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5844300/ https://ncbi.nlm.nih.gov/pubmed/29321167 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.117.300363 |
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