Structure of the 30 kDa HIV-1 RNA Dimerization Signal by a Hybrid Cryo-EM, NMR and Molecular Dynamics Approach

Electron cryo-microscopy (cryo-EM) and NMR spectroscopy are routinely used to determine structures of macromolecules with molecular weights over 65 kDa and under 25 kDa, respectively. We combined these techniques to study a 30 kDa HIV-1 Dimer Initiation Site RNA ([DIS](2); 47 nt/strand). A 9 Å cryo-...

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Udgivet i:Structure
Main Authors: Zhang, Kaiming, Keane, Sarah C., Su, Zhaoming, Irobalieva, Rossitza N., Chen, Muyuan, Van, Verna, Sciandra, Carly A., Marchant, Jan, Heng, Xiao, Schmid, Michael F., Case, David A., Ludtke, Steven J., Summers, Michael F., Chiu, Wah
Format: Artigo
Sprog:Inglês
Udgivet: 2018
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5842133/
https://ncbi.nlm.nih.gov/pubmed/29398526
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.str.2018.01.001
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